Figure 7.

Alignment of the SmiCI and SpnCI integrases that target either manA or uvrA. The integrase proteins encoded by the S. mitis SmiCI B6, the S. mitis SmiCI SK1080, and S. pneumoniae Hungary19A-6 SpnCI were aligned using ClustalX (Jeanmougin et al., 1998). The integrases from SpnCI Hungary19A-6 and SmiCI SK1080 both recognize an identical attachment site in the promoter of uvrA, while the SmiCI B6 integrase recognizes manA. Above the alignment, an asterisk (^*) indicates identity between the three proteins, a colon (:) indicates a strongly conservative amino acid substitution, and a dot (.) indicates a weakly conservative amino acid substitution