Table 2.

Folding thermodynamics of proteins 8–13 from circular dichroism measurements^a

Protein	Tm (°C)a	ΔΔGfold vs. 8 (kcal mol−1)	substitutions vs. 8	ΔΔGfold per substitution (kcal mol−1)
8	82.1
9	75.6	+1.1	2 α→N-Me-α	0.6
10	43.5	+6.3	4 α→γ4	1.6
11	46.7	+5.9	4 α→γcyc	1.5
12	78.0	+0.7	4 side chains	0.2
13	74.3	+1.3	4 α→γcyc	0.3

^aCD experiments carried out in pH 7 phosphate buffer