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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: J Cell Physiol. 2014 Nov;229(11):1822–1830. doi: 10.1002/jcp.24636

Figure 2. L. reuteri treatment prevents Ovx-induced increases in RANKL and bone marrow osteoclastogenesis after 2 weeks of probiotic treatment.

Figure 2

Conditions are non-Ovx, intact mice (white bar); Ovx, ovariectomized mice (gray bar); Ovx + Lr, Ovx mice gavaged with L. reuteri for 2 weeks (black bar). (A) Levels of TRAP5 and RANKL RNA in whole tibia bone. Levels were expressed relative to HPRT, a non-modulated house-keeping gene. (B) Harvested mouse bone marrow cells from the mice above were cultured in vitro with RANKL and M-CSF and the number of osteoclasts counted per well. (C) Osteocalcin mRNA levels were measured in whole tibia bone and expressed relative to HPRT. Data values are average ± standard error, n ≥ 8 per group, * p<0.05 to Non-Ovx, ˆ p<0.05 to Ovx by one-way ANOVA followed by Tukey HSD.