Table 1.
Genes chosen for qPCR screen
|
Predicted hsa-mir-30c targets from microRNA.org | ||
|---|---|---|
| Gene symbol | mirSVR Score | References into function or ontology terms |
| TWF1 |
−3.02 |
GO: Actin Binding |
| Regulates actin filament turnover [31] | ||
| Promotes cellular motility through actin regulation [32] | ||
| DYNLT3 |
−3.02 |
GO: Cytoplasm, GO: Plasma Membrane |
| Cytoplasmic dynein light chain protein that interacts with spindle checkpoint protein BUB3 [33] | ||
| Functions as a nuclear matrix-associated transcription factor in a dynein-independent manner [34] | ||
| NEDD4 |
−2.47 |
Modulates p-Smad1 signaling in response to both BMP-2 and TGFβ1 [35] |
| Negatively regulates PTEN in an oncogenic fashion [36] | ||
| Involved in the deposition of extracellular collagen [37] | ||
| PTPN3 |
−2.55 |
Regulates EGF-mediated cell-cell contact [38] |
| Regulates focal adhesion [39] | ||
| Regulates Cadherin-mediated cell-cell contact [40] | ||
| Regulates neural crest cell adhesion and motility [41] | ||
| Controls hormone receptor signaling [42] | ||
| Cooperates with vitamin D to promote breast cancer cell growth [43] | ||
| ADAM22 |
−2.44 |
GO: Extracellular, GO: Metallopeptidase Activity |
| Metalloproteinase-like, disintegrin-like, cysteine-rich protein that is highly expressed in brain tissue [44] | ||
| Promotes cell adhesion and spreading [45] | ||
| Cooperates with SRC-1 in endocrine resistance of breast cancer cells; expression of ADAM22 independently predicts poor survival in patients [46] | ||
| NOV |
−3.11 |
Regulates actin cytoskeletal reorganization [28] |
| Increases motility of chondrosarcoma cells [47] | ||
| Promotes mineralization of osteoblasts [48] | ||
| Transcriptionally activated by p53 [24] | ||
| Downregulated in advanced melanomas [26] | ||
| Downregulated in childhood adrenocortical tumors [25] | ||
| Significantly over-expressed in ER-positive breast cancer patients who relapsed after tamoxifen treatment [49] | ||
| Expression is negatively correlated with metastasis and progression in breast cancer [50] | ||
| Promotes xenograph breast cancer bone-metastasis and osteolysis [27] | ||
| CELSR3 | −4.03 | GO: G-protein coupled receptor signaling pathway, GO: neuron migration, and GO: plasma membrane |
Genes were chosen for qPCR screen based on: mirSVR score (www.microrna.org) [21], NCBI GeneRIF [22], and ontological terms [23]. TWF1 references: [31],[32]. DYNLT3 references: [33],[34]. NEDD4 references: [35]–[37]. PTPN3 references: [38]–[43]. ADAM22 references: [44]–[46]. NOV references: [24]–[28],[47],[48],[50].