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. 2014 Aug 5;107(3):682–693. doi: 10.1016/j.bpj.2014.05.044

Figure 3.

Figure 3

Comparison of N-cTnC Cys-13–Cys-51 distance distributions as a function of cTnC site-II Ca2+-occupancy and cross-bridge state when observed at short 1.8-μm SL in cTnC(T13C/N51C)AEDANS-DDPM-reconstituted myocardial fibers. (A) Normalized distance distributions obtained using global-curve analysis of intensity decay profiles observed under the following conditions: pCa 9 + ATP (black solid line), pCa 4.3 + ATP (black dotted line), pCa 9 + ADP (orange solid line), pCa 4.3 + ADP (orange dotted line), pCa 9 + Vi (green solid line), and pCa 4.3 + Vi (green dotted line). (Area-normalized versions of these distance distributions are shown in Fig. S6.) (B) As a function of whether Ca2+ is bound to N-cTnC and at 1.8 μm SL, this panel shows the statistical significance of differences in r and HWHM values that occurred because of changes in cross-bridge binding state. (C and D) Significance of differences in r and HWHM due to reduction of SL. The number of determinations was 5–6 for each group. ∗p < 0.05; ∗∗p < 0.005; ∗∗∗p < 0.001.