. 2014 Aug 5;107(3):682–693. doi: 10.1016/j.bpj.2014.05.044

Table 2.

Cys-13–Cys-51 distance distributions observed in cTnC(N13C/T51C)_AEDANS-DDPM-reconstituted fibers under different biochemical conditions

Cross-bridge state	pCa	r^a (Å)		HWHM^b (Å)
Cross-bridge state	pCa	1.8 μm SL	2.2 μm SL	1.8 μm SL	2.2 μm SL
5 mM ATP	9.0	26.21 ± 0.27	25.53 ± 0.29	6.23 ± 0.64	7.19 ± 0.07
5 mM ATP	4.3	31.42 ± 0.42	32.97 ± 0.25	5.52 ± 0.42	8.05 ± 0.37
1 mM Vi^a	9.0	25.46 ± 0.12	25.07 ± 0.10	5.11 ± 0.62	5.52 ± 0.20
1 mM Vi^a	4.3	29.97 ± 0.40	29.83 ± 0.31	5.15 ± 0.31	5.85 ± 0.26
5 mM ADP^b	9.0	25.70 ± 0.39	26.72 ± 0.16	5.74 ± 0.45	4.39 ± 0.19
5 mM ADP^b	4.3	31.80 ± 0.14	33.78 ± 0.08	5.48 ± 0.56	3.78 ± 0.71
0.5 mM Vi^a	9.0	—	25.75 ± 0.19	—	6.57 ± 0.12
0.5 mM Vi^a	4.3	—	32.03 ± 0.18	—	5.43 ± 0.43
2.5 mM ADP^b	9.0	—	26.39 ± 0.26	—	5.18 ± 0.10
2.5 mM ADP^b	4.3	—	33.28 ± 0.22	—	6.41 ± 0.37

Absolute parameter values are given as mean ± SE.

Vi solutions also contained 5 mM ATP.

ATP was absent in ADP solutions.