Table 1.

Energy of hydrogen bonds in the Long-AB interface

TUB 1-α	TUB 2-β	Eaverage	SD
Arg2	Glu71	–40.1	14
Glu434	Arg401	–32.6	17
Tyr262	Arg401	–32.1	17
Arg243	Asp76	–29.9	14
Thr349	Val181	–25.8	12
Asp438	Arg401	–23.9	25
Val260	His406	–22.1	11
Gln133	Gly98	–21.9	8
Thr257	Gly100	–21.4	7
Lys352	Thr180	–18.7	9
Asn249	Gln11	–18.6	11
Asn329	Lys176	–15.3	10
Lys163	Glu411	–14.7	19
Asn258	Val181	–13.9	8
Other bonds		–61.3	—
Total energy		–392	59

The Long-AB interface refers to two αβ-tubulin heterodimers aligned longitudinally (TUB 1 and TUB 2). Average energy and standard deviation (SD) values are taken from eight different representative snapshots. Energies are expressed in kJ/mol.