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. 2014 Aug 5;107(3):700–710. doi: 10.1016/j.bpj.2014.06.023

Figure 2.

Figure 2

Molecular structures of the fluorophores used in this work. (A) Canonical structure of the donor, ATTO 610, conjugated to a C-6 linker. (B) Structure of the acceptor dye, ATTO 647N, attached to the same linker shown in (A). (C) Geometry-optimized structures of fluorophore ring systems corresponding to the donor excited state and acceptor ground state obtained from ab initio quantum-mechanical calculations. The directions of the transition-dipole moments are indicated (arrows). We define R as the distance between the centers of mass of rigid three-ring conjugated systems corresponding to each dye. (One column; 3.25″ W × 2.34″ H.) To see this figure in color, go online.