Table 3.

Kinetic parameters of the model

Parameter	Description	Parameter value	Experimental value	Source
k0+ATP	ATP binding	2 × 106 M−1 s−1	2.2 × 106 M−1 s−1	(58)
k0−ATP	ATP release	50 s−1	175 s−1a	(58)
k0−MT	MT release, D	500 s−1	460 s−1	(26)
k0+MT	MT binding, D	100 s−1
k′0+RS	ATP hydrolysis, linker swing to prestroke	1000 s−1	180 s−1b	(58)
k′0−RS	ATP synthesis, linker swing to poststroke	100 s−1	4 s−1; 30 s−1	(47,58)
k′0+MT	MT binding, D∗	10,000 s−1
k′0−MT	MT release, D∗	500 s−1
k0−Pi	Pi release	5000 s−1
k0+Pi	Pi binding	104 M−1 s−1	8000 M−1 s−1c	(47)
k0+PS	Power stroke	5000 s−1
k0−PS	Reverse stroke	10 s−1
k0−ADP	ADP release	160 s−1	∼1000 s−1	(27)
k0+ADP	ADP binding	2.7 × 106 M−1 s−1	2.7 × 106 M−1 s−1	(27)

^aCalculated from K_eq and K_+ATP.

^bMeasurement includes the transition between ATP binding and isomerization.

^cWithout MT.