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. Author manuscript; available in PMC: 2015 Jun 5.
Published in final edited form as: Cell. 2014 Jun 5;157(6):1292–1308. doi: 10.1016/j.cell.2014.03.066

Figure 1. Type 2 cytokines IL-4/13 are required for biogenesis of cold-induced beige fat.

Figure 1

(A) Quantitative RT-PCR analysis of thermogenic genes in scWAT of WT and Il4/13-/- mice housed at 30°C, 22°C, or 5°C for 48 hours (n=5 per genotype and temperature). (B) Immunoblot analysis of UCP1 in scWAT and BAT of WT and Il4/13-/- mice housed at 22°C or 5°C for 48 hours (n=3 per genotype and temperature). (C, D) Representative scWAT sections of WT and Il4/13-/- mice housed at 30°C, 22°C, or 5°C for 48 hours were stained with hematoxylin and eosin (C) or for UCP1 (D), scale bar 100 μm. (E, F) Oxygen consumption in scWAT (E) and BAT (F) from WT and Il4/13-/- mice housed at 22°C or 5°C for 48 hours (n=7-8 per genotype and temperature). (G-I) Oxygen consumption (VO2) in WT and Il4/13-/- mice at different environmental temperatures: at thermoneutrality, 30°C (G) or at different ambient temperatures (H, I), n=5-8 per genotype. Data are represented as mean ± SEM. See also Figure S1.