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. Author manuscript; available in PMC: 2015 Jun 5.
Published in final edited form as: Cell. 2014 Jun 5;157(6):1292–1308. doi: 10.1016/j.cell.2014.03.066

Figure 4. Macrophage recruitment via CCR2 and alternative activation via IL-4Rα is required for biogenesis of beige fat.

Figure 4

(A) Ly6Chi macrophage content of scWAT in WT and Ccr2-/- mice housed at thermoneutrality (30°C) or at 5°C for 48 hours (n=5 per genotype and temperature). (B, F) UCP1 protein expression in WT and Ccr2-/- mice (B) or Il4raf/f and Il4raf/fLyz2Cre (F) mice at 22°C or 5°C (n=3 per genotype and temperature). (C, D) Cold-induced changes in oxygen consumption (VO2) in WT and Ccr2-/- mice (n=8 per genotype). Data are represented as mean ± SEM. (E) Expression of alternative activation marker Arginase 1 in scWAT macrophages of WT, Il4/13-/-, and Il4ra-/- mice housed at 30°C or 5°C (n=4-5 per genotype and temperature). (G, H) Oxygen consumption in scWAT (G) and BAT (H) from Il4raf/f and Il4raf/fLyz2Cre mice housed at 22°C or 5°C for 48 hours (n=6 per genotype and temperature). (I-K) Oxygen consumption (VO2) in Il4raf/f and Il4raf/fLyz2Cre mice at various environmental temperatures: thermoneutrality, 30°C (n=5 per genotype) or 22°C and 4°C (n=7-9 per genotype). (L) Adipose tissue weights of Il4raf/f and Il4raf/fLyz2Cre mice after cold challenge at 5°C for 48 hours (n=5 per genotype). (M) Core body temperature of Il4raf/f and Il4raf/fLyz2Cre mice during a 48 hour cold challenge (n=5 per genotype). Data are represented as mean ± SEM. See also Figures S3 and S4.