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. Author manuscript; available in PMC: 2015 Apr 16.
Published in final edited form as: Neuron. 2014 Mar 27;82(2):308–319. doi: 10.1016/j.neuron.2014.02.027

Figure 3. Aβ injected to wt hippocampus in vivo is recovered from the membrane-associated fraction as bound to GM1 ganglioside.

Figure 3

320 pg or 320 ng synthetic S26C Aβ dimers, huAD TBS dimers or buffer (mock) were injected to anesthetized wt mice, then the brains were immediately harvested for brain fractionation. The in vivo injected brain fractions (TBS and TBS-Tx extracts) or S26C Aβ spiked ex vivo to extracts or to buffer alone were immunoprecipitated for Aβ using R1282. WB: 3D6+6E10 for Aβ (A) or CTβ for GM1 (B). Representative blots of N=5, 3, 5 and 5 mice for 320 pg S26C, 320 ng S26C, mock or huAD TBS dimer injections, respectively. See also Figure S3.