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. Author manuscript; available in PMC: 2015 Apr 16.
Published in final edited form as: Neuron. 2014 Mar 27;82(2):308–319. doi: 10.1016/j.neuron.2014.02.027

Figure 7. Low levels of GM1-bound Aβ are recovered from hAPP tg mouse ISF and human CSF.

Figure 7

(A-C) IP: 4396C, WB: CTβ for GM1 ganglioside or WB: 3D6+6E10+266+2G3+21F12 for Aβ. (A, B) 4396C pull-down of human CSF probed for Aβ then stripped and reprobed for GM1 (A) or GM1 alone on a naïve IP blot (B; Red asterisk=GM1-Aβ). (C) CSF from 2 humans with differing levels of Aβ42 was immunoprecipitated for GM1-bound Aβ. Representative blots of at least 3 experiments. (D, E) Levels of GM1-bound Aβ recovered using 4396C in ten human CSF samples showed a strong correlation to their levels of Aβ42 (D; 21F12/3D6B ELISA) and less but still significant to levels of Aβ40 (E; 2G3/3D6B ELISA).