Mice were injured at T9 and sacrificed at 1, 3, and 7 days post-injury (DPI). Samples were processed for immunofluorescence staining or Western blot analysis. A. Representative images from sham (S) and 1, 3, and 7 DPI. PS1, a component of γ-secretase, is increased in the epicenter as well as the sections rostral and caudal to the injury site (Mag. Bar = 500 µm). The thumbnail image shows the no primary antibody negative control. B. The quantitation of immuno fluorescence data using image J (n=3), shows a significant increase in PS1 at 1 and 3 DPI as compared to sham in all areas evaluated. C. Western blot data confirms the increase of PS1 after SCI (n=3). D. Western blot data demonstrate that Bace1 is significantly increased at 1 day after injury (n=3).