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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: J Tissue Eng Regen Med. 2012 Aug 17;8(8):629–639. doi: 10.1002/term.1564

Figure 1. Experimental design.

Figure 1

Groups 1 & 2 are controls, where constructs were provided osteogenic supplements (OM) or endothelial factors (EGM) for 5 weeks. Group 3 was cultured with a cocktail of both OM & EGM (1:1) for 5 weeks. In Groups 4 & 5, vascular differentiation was induced for 2 weeks before adding osteogenic factors in a cocktail medium (EGM∣cocktail). Additional hASC were added into the pore spaces at this point in Group 5 (EGM∣cocktail+ASC). Groups 6 & 7 were seeded with hASC to silk scaffold and osteogenic differentiation was induced for 3 weeks; only at this time point, hASC in fibrin were added to the scaffold for vascular development. Constructs were cultured in either EGM (Group 6 – OM∣EGM) or cocktail media (Group 7 – OM∣cocktail) for the remaining 2 weeks.