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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: J Tissue Eng Regen Med. 2012 Aug 17;8(8):629–639. doi: 10.1002/term.1564

Figure 4. Immunohistological analysis of constructs after 5 weeks.

Figure 4

The expression of vascular and osteogenic proteins was evaluated. CD31 and von Willebrand Factor (vWF) were expressed in endothelial control group (EGM) as well as in both groups where endothelial differentiation was firstly induced (EGM∣cocktail & EGM∣cocktail+ASC). These proteins were also detected in the group where EGM was supplemented after osteogenic differentiation (OM∣EGM). Osteopontin (OPN) and bone sialoprotein (BSP) were expressed in the matrix of all groups with exception to EGM group, where these proteins were detected only at a cellular level. Scale bar = 100 μm.