. Author manuscript; available in PMC: 2014 Aug 12.

Published in final edited form as: Nat Chem Biol. 2010 Dec 26;7(2):101–105. doi: 10.1038/nchembio.499

Table 1. Suppression of Growth Defect of WP758 ΔproBA cells expressing proB and proA variants from the plasmid pBAD24.

Single colonies were streaked on MOPS_casein minimal medium agar plates containing 0.2 % arabinose (to induce expression from araBAD promoter in pBAD24 plasmid), ampicillin, and 0.1 mM AsO₄^3-. Growth was examined after 15 hours. – indicates no growth, + indicates good growth, and +/- forms tiny colonies.

Plasmid	Growth of WP758 ΔproBA on AsO₄^3-
pBAD24-proB proA	-
pBAD24-proB(A117V) proA(S362F)	+
pBAD24-proB(T120I) proA(S362F)	+
pBAD24-proB(E143K) proA(R143C)	+
pBAD24-proB(A117V) proA	+/-
pBAD24-proB proA(S362F)	-
pBAD24-proB(T120I) proA	+/-
pBAD24-proB(E143K) proA	+/-
pBAD24-proB proA(R143C)	-