Table 1.
Target gene | PCR primer sequence | PCR product (bp) | |
---|---|---|---|
GAPDH | Forward | 5′-GGCATTGCTCTCAATGACAA-3′ | 223 |
Reverse | 5′-TGTGAGGGAGATGCTCAGTG-3′ | ||
TNF-α | Forward | 5′-ACTCCCAGAAAAGCAAGCAA-3′ | 211 |
Reverse | 5′-CGAGCAGGAATGAGAAGAGG-3′ | ||
IL-1β | Forward | 5′-AGGCTTCCTTGTGCAAGTGT-3′ | 230 |
Reverse | 5′-TGAGTGACACTGCCTTCCTG-3′ | ||
IL-6 | Forward | 5′-CTGCTCTGGTCTTCTGGAGT-3′ | 229 |
Reverse | 5′-GGTCTTGGTCCTTAGCCACT-3′ | ||
IL-10 | Forward | 5′-ATAACTGCACCCACTTCCCA-3′ | 231 |
Reverse | 5′-TTTCTGGGCCATGGTTCTCT-3′ |