Table 2.
Relative retention times (RRTs) and concentrations (ng/g) measured for AEG, BMAA and DAB in samples and in Cycad control material as analyzed by the RPLC-MS/MS method
| RRTs* | Concentrations (ng/g)** | |||||
|---|---|---|---|---|---|---|
| Sample # |
AEG |
BMAA |
DAB |
AEG |
BMAA |
DAB |
| GH-1 |
nd |
nd |
1.08 |
nd |
nd |
1226 |
| GH-2 |
0.96 |
nd |
1.09 |
290 |
nd |
2493 |
| GH-3 |
0.96 |
nd |
1.09 |
191 |
nd |
1773 |
| GH-4 |
0.96 |
nd |
1.09 |
569 |
nd |
2569 |
| GH-5 |
0.96 |
nd |
1.09 |
1348 |
nd |
874 |
| GH-6 |
0.96 |
nd |
1.09 |
1240 |
nd |
1599 |
| GH-7 |
0.96 |
nd |
1.09 |
1136 |
nd |
963 |
| GH-8 |
0.96 |
nd |
1.09 |
486 |
nd |
490 |
| GH-9 |
0.96 |
nd |
1.09 |
290 |
nd |
1270 |
| GH-10 |
0.96 |
nd |
1.09 |
500 |
nd |
696 |
| Cycad (NRC) | 0.96 | 1.003 | 1.08 | 375 | 208,089 | 631 |
*relative to d3-BMAA (RT = 6.60 min).
**internal standard quantitation was used for BMAA, while external calibration.
nd = not detected (LOD = 80 ng/g for BMAA).
Retention times are expressed relative to a d3-BMAA internal standard that was spiked into the individual samples.