Table 1.
Virus-specific primers used in this study.
| Virus | GenBank acc. number | Primer sequences | Position |
|---|---|---|---|
| HERVWE1 | |||
| Envelope (=syncytin) | NM_014590 | F: cat cga tag cac cca tca gat g | 13–34 |
| R: gag tga aat agc atg aaa aca g | 3025–3004 | ||
| ERVWE1 gag | AF156961 | F: tgt ccg ctg tgc tcc tga tc | 1–20 |
| R: ctg cgc cag tgt cca gga gac | 1921–1901 | ||
|
| |||
| HERVWE2 | |||
| Envelope | AF127228, | F: cca ata gcc aga cca tta tat ac | 1–23 |
| AF128229, | R: tgg ggt tcc att tgt aag acc | 1932–1912 | |
| ERVWE2 gag | AF123881 | F: cta gaa cgt att ctg gag aat tg | 2–24 |
| R: ggc tct caa tgg tca aac ata c | 1509–1488 | ||
|
| |||
| HHV-1 | GU734772 | F: tcc cca taa act ggg agt agc | 55192–55212 |
| R: cag aac tac agc gag ggc atc | 55327–55307 | ||
|
| |||
| HHV-6 | NC_000898 | F: gag tcc atg agt tag aag att | 150874–150894 |
| R: cta aat ttt cta cct ccg aaa tgt | 152099–152076 | ||
|
| |||
| HHV-4 (Epstein Barr) | V01555 | External primers | |
| F: agg gat gcc tgg aca caa ga | 48810–48829 | ||
| R: tgg tgc tgc tgg tgg tgg caa t | 49406–49385 | ||
| Nested primers | |||
| F: tcttgatag gga tcc gct agg ata | 48839–48862 | ||
| R: acc gtg gttbctg gac tat ctg gat | 49335–49311 | ||
|
| |||
| HHV-5 (human cytomegalovirus) | GQ222016 | External primers | |
| F: cag cac cat cct cct ctt cct ctg | 3665–3688 | ||
| R: cca agc ggc ctc tga taa cca agc | 4099–4076 | ||
| Nested primers | |||
| F: aga cac tgg ctc aga cct gac | 3708–3728 | ||
| R: aga gtc tgc tct cct agt gtg | 3989–3969 | ||
|
| |||
| Hepatitis C virus | AB691598 | External primers | |
| F: ggc gac act cca cca tgg atc ac | 16–38 | ||
| R: cat gtt gca cgg tct acg aga cc | 342–320 | ||
| Nested primers | |||
| F: ctg tga gga act tct gtc tt | 43–62 | ||
| R: ctc gca agc acc cta tca gg | 309–290 | ||
|
| |||
| Human parvovirus B19 | NC_000883 | External primers | |
| F: aat aca ctg tgg ttt tat ggg ccg | 1579–1602 | ||
| R: cca ttg ctg gtt ata acc aca ggt | 1862–1839 | ||
| Nested primers | |||
| F: aat gaa aac ttt cca ttt aat gat gta g | 1678–1705 | ||
| R: cta aaa tgg ctt ttg cag ctt cta c | 1780–1756 | ||
|
| |||
| Rabbit endogenous retrovirus (HRV5) | AF480924 | External primers | |
| F: tca ggt gct tca ttg gca gga tca | 3008–3031 | ||
| R: taa aat ttg tac ttt tgg gca ctg ctg | 3805–3782 | ||
| Nested primers | |||
| F: tgc aac ctt atg tta gtg cac tcc | 3052–3075 | ||
| R: tac tgc ctg gtc aac ata tag | 3766–3746 | ||