Figure 1.

Discrimination of endogenous lipofuscin granules and melanosomes in a human RPE cell. Bright field imaging (A) shows all granules, and fluorescence imaging (B) of the same cell identifies autofluorescent lipofuscin granules. In the cell periphery, where granules are less numerous, individual granules can be discriminated on merged images (C). A magnified region (D) illustrates an adjacent melanosome (black arrow) and lipofuscin granule (white arrow). Note that the two images of the lipofuscin granule do not align due to the granule's movement between capture of sequential bright field and fluorescence images. N, nucleus. Age of RPE donor: 84 years. Magnifications: (A–C) ×400, (D) ×600.