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. 2014 Jul 22;2014:729618. doi: 10.1155/2014/729618

Table 1.

Immunogenic CW proteins of MAP identified by MALDI-TOF MS. Proteins were excised from Coomassie blue stained gels and subsequently identified by MALDI-TOF at the Mass Spectrometry Center for Biological and Chemical Analysis (CEQUIBIEM) at the School of Exact and Natural Sciences, University of Buenos Aires. ND: no data.

Spot number MAP Locus Mtb Locus Gene Protein function/family ORF size (bp) Previously described as envelope protein
1 MAP1164 Rv1436 gapdh Glyceraldehyde-3-phosphate dehydrogenase 1,020 He and de Buck, 2010 (MAP) [34]
2 MAP1205 Rv1479 moxR ATPase 1,143 Mawuenyega et al., 2005 (Mtb) [35]
3 MAP1325 Rv1630 rpsA S1 30S ribosomal protein S1 1,443 Gu et al., 2003 (Mtb) [36]
4 MAP1367 Rv1658 argG Argininosuccinate synthase 1,197 ND
5 MAP1889c Rv2145c wag31 DivIVA family protein 783 He and de Buck, 2010 (MAP) [34]
6 MAP1962 Rv2220 glnA1 Glutamine synthetase A1 1,437 Gu et al., 2003 (MAP) [36]
7 MAP1998 Rv2245 kasA 3-Oxoacyl synthetase 1,251 He and de Buck, 2010 (MAP) [34]
8 MAP1999 Rv2246 kasB_1 3-Oxoacyl sintase 2 B 1,323 Mawuenyega et al., 2005 (Mtb) [35]
9 MAP2453c Rv1308 atpA ATP synthase subunit alpha 1,665 He and de Buck, 2010 (MAP) [34]
10 MAP2855c Rv2744c 35kd_ag Phage shock protein A 828 ND
5 MAP3152c Rv3075c hpcH/hpaI Aldolase/citrate lyase 921 Gu et al., 2003 (MAP) [36]
11 MAP3404 Rv3285 accA3 Carbamoyl-phosphate synthase subunit A 1,824 Mawuenyega et al., 2005 (Mtb) [35]
12 MAP3567 Rv0148 Hypothetical protein Short-chain dehydrogenases/reductases 864 He and de Buck, 2010 (MAP) [34]
13 MAP3651c Rv0215c fadE3_2 Acyl-CoA dehydrogenase FadE3 1,218 He and de Buck, 2010 (MAP) [34]
7 MAP3692c Rv0242c fabG4 3-Ketoacyl reductase 1,365 He and de Buck, 2010 (MAP) [34]
14 MAP3853 Rv0384c clpB ATP dependent protease ClpB 2,547 He and de Buck, 2010 (MAP) [34]
3 MAP3936 Rv0440 groEL2 GroEL chaperonin 1,626 He and de Buck, 2010 (MAP) [34]
15 MAP4143 Rv0685 eftu Elongation factor Tu 1,191 He and de Buck, 2010 (MAP) [34]