Table 3.

Reactivity of bovine sera to the protein EF-Tu by line print immunoassay. 20 μL of antigens was applied onto a nitrocellulose membrane and simultaneously confronted to sera from 10 healthy animals, 8 animals with bovine tuberculosis (TBB), and 25 animals with paratuberculosis (PTB). Sixty four percent of MAP positive sera recognized EF-Tu.

Number of sera with positive recognition
Antigen	Healthy (n = 10)	PTB infected (n = 25)	TBB infected (n = 8)
PPDA	4	16	3
PPA-3	1	18	2
PPDB	6	1	4
EF-Tu	8	16	7