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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: J Bone Miner Res. 2014 Mar;29(3):666–675. doi: 10.1002/jbmr.2075

Figure 2.

Figure 2

Sc65 expression during mouse embryonic development. A–C In situ hybridization showing Sc65 mRNA expression (red signal) in mouse embryo sections of ribs at E16.5 (A) and in metatarsus (B) and tibia (C) at P2. D,G Hematoxilin and eosin stained sections of E13.5 rib and E15.5 metatarsus, respectively. E–F, H–I Serial sections of D and G stained with Sc65 antibody (E and H) or secondary antibody only (F and I). J. Qualitative Western Blot on several P3 tissue lysates. PC = positive control (osteoblast lysate), NC= negative control (Sc65Mut osteoblast lysate), Cal = calvaria, Br = brain, Liv = liver, Kid = kidney, Hrt = heart, Msc = muscle, Sk =skin, Cart = cartilage. The boxed region identifies Sc65.