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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: J Bone Miner Res. 2014 Mar;29(3):666–675. doi: 10.1002/jbmr.2075

Figure 4.

Figure 4

Increased osteoclastogenesis in Sc65 mutant mice. Ex-vivo whole bone marrow (A) and bone marrow macrophages (BMM) (B) cultures (n=3 each) from 5 month-old or older mice showed increased number of TRAP-positive multinucleated cells (TRAP+ MNC/well) in Sc65Mut compared to WT mice (C). Serum levels of CTX were significantly elevated in 6-month old Sc65Mut compared to WT mice (n = 7, p = 0.012). Increased numbers of TRAP-positive MNCs (D) and resorption areas (E) were observed when Sc65Mut splenocytes or BMMs were plated either on plastic (96-well plate), osteo-assay plates or dentine slices, compared to WT controls (F). Quantification of resorption pits area from Osteassay plates (p = 0.015).