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. Author manuscript; available in PMC: 2014 Aug 12.
Published in final edited form as: Microbiology (Reading). 2009 Feb;155(Pt 2):566–575. doi: 10.1099/mic.0.024620-0

Figure 4. Effect of mutations on the binding of CovR and CovR-P to Pska: mutations in CB-1 and CB2.

Figure 4

Lanes 1–4:wild type DNA. Lanes 5–8: Mutant DNA with a TT to GG transversion in site CB-1 (CB-1*). Lanes 9–12: Mutant DNA with a TT to GG transversion in site CB-2 (CB-2*). Lanes 1–4, lanes 5–8 and lanes 9–12; phosphorylated CovR at 0, 0.25, 0.5, 1 and 3 μM respectively. The location of the CovR binding sites CB1 and CB2 are indicated at the right of the figure. The solid line indicates a region of DNaseI protection. The arrow indicates the direction of transcription.