Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Yuh1 site-specifically modifies Ub dimers. 48-linked di-Ub (700 µM) was incubated with I (250 mM) and Yuh1 (150 nM) overnight.(A) Experimental scheme. (B) Biotinylated dimers are detected by their interaction with TAMRA-labeled streptavidin. Fluorescence was detected with a green laser (532 nm) and a 580±15 nm filter on a Typhoon imager FLA 9500 (GE Healthcare). Coomassie-stained SDS-PAGE is shown for the purposes of a loading control. NEM; N-ethylmaleimide.
