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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Radiat Res. 2014 Jun 19;182(1):102–110. doi: 10.1667/RR13635.1

FIG. 1.

FIG. 1

Effect of increasing doses of gamma radiation on plasmid (pBR322) DNA relaxation. Supercoiled (SC) represents the compact form and open-circular (OC) represents the relaxed or damaged form of the plasmid DNA. Panel A: The SC form is seen as the lower prominent band (at 3,000 bps) while the OC form is the upper prominent band. Lane 1: 1 kb DNA standard ladder; lanes 2 and 3: untreated plasmid DNA; lanes 4 and 5: plasmid DNA exposed to 10 Gy of radiation; lanes 6 and 7: plasmid DNA exposed to 25 Gy of radiation; and lanes 8 and 9: plasmid DNA exposed to 50 Gy of radiation. Panel B: SC and OC forms are presented as percentage of total plasmid DNA. For each condition, all samples were run in duplicate. The data are presented as mean ± standard deviation. P < 0.05 was considered significant. *Indicate a significant difference compared to untreated *SC and **OC forms.