Figure 4.

Dynamics of native backbone H-bonds (see Table 2 for peptide names, and Figure 3 for Buckling, Near-eq , Hyper-ela, and Free). (a) Occupancy and (b) average lifetime. Standard deviations of lifetimes are in Supporting Information, Figure S7a. Measured values with glycines as H-bond donors are averaged for each triad and represented in color. Triads 11–14 contain cleavage sites (solid box). The imino-poor domain spans triad 10–20. Compared to other peptides, H-bonds of gpo10 have notably smaller occupancy, lifetime, and standard deviation (Supporting Information, Figure S7a), suggesting a dynamic stabilization mechanism.