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. 2013 Dec 27;10(3):640–647. doi: 10.4161/hv.27576

graphic file with name hvi-10-640-g4.jpg

Figure 4. Intracellular interferon-γ (IFN-γ), interleukin 4 (IL-4), and interleukin 17 (IL-17) staining of CD3+ CD4+ cells isolated from spleens. Initial gating was performed using the CD4 marker. The percentage of IL-4 secreted cells in control and experimental groups are shown in panel (A). The percentage of IL-17 secreted cells in control and experimental groups are shown in panel (B). The percentage of IFN-γ secreted cells in control and experimental groups are shown in panel (C). Wd, wild type; KO, C3 knockout.