Figure 2. Expression of sPD-1 and sTim-3 mediated by rAd5-sPD1 and rAd5-sTim3. (A) Schematic presentation of rAd5-sPD1 and rAd5-sTim3 carrying genes encoding sPD-1 and sTim-3. The coding sequences of sPD-1 and sTim-3 contain the signal sequence and the extracellular domain of PD-1 and Tim-3 protein. The coding sequences of sPD-1 and sTim-3 were cloned into the E1 region of adenoviral vector to obtain recombinant adenoviruses rAd5-sPD1 and rAd5-sTim3 respectively. (B) Expression of sPD-1 and sTim-3 proteins by rAd5-sPD1 and rAd5-sTim3. Vero cells were infected with rAd5-sPD1, rAd5-sTim3, or rAd5-empty respectively. At 48 h post-infection, the culture media were subjected to SDS-PAGE followed by western blot analysis using rabbit antibodies specific for sPD-1 and sTim-3.