Table 1A. The table for LD₅₀ calculation.

Group	Dilution fold	Dose (CCID50/Dose)	Log Dose	No.of Dead	No. of survival	Cumulative			Mortality rate	% mortality
						Dead	Survival	Total
1	1000000	0.0016	−2.8010	0	8	0	18	18	0/18	0.00%
2	100000	0.0158	−1.8010	2	6	2	10	12	2/12	16.67%
3	10000	0.1581	−0.8010	5	4	7	4	11	7/11	63.64%
4	1000	1.5811	0.1990	6	0	13	0	13	13/13	100.00%
5	100	15.8114	1.1990	7	0	20	0	20	20/20	100.00%

Estimation of median percent mortality, (MLD₅₀); (50.0–16.67) ÷ (63.64–16.67) = 33.33 ÷ 46.97 = 0.7096; (−0.80103) – (−1.80103) = 1.0; ∴0.7096*1.0 = 0.7096; Antilog of −1.80103 + 0.7096 = Antilog of −1.09143; LD₅₀ = 0.081 CCID₅₀; Table 1B.