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. Author manuscript; available in PMC: 2014 Aug 12.
Published in final edited form as: Mol Biochem Parasitol. 2007 Nov 1;157(2):160–168. doi: 10.1016/j.molbiopara.2007.10.009

Figure 3.

Figure 3

Induction of specific RNAi following treatment with cathepsin D dsRNA. (A) SmCD enzyme activity in extracts of schistosomula. Three hour old schistosomula were electroporated with 10 μg of SmCD 1.2 kb-dsRNA or Luc-dsRNA, and cultured for 14 days post-electroporation in the presence of human erythrocytes. (B) Shows relative cathepsin D gene expression of the same treatment group. RNA was 10 fold serially diluted, and assayed by RT-PCR for SmCD and SmCB1. The dilution series reflects input of 50.0, 5.0, 0.05 ng of total RNA into the RT-PCRs. In all cases, statistically significant differences between experimental and control groups were observed (p<0.05).