Table 2. Cytokine levels correlate with cell type in preterm lavage.
IL-1β | CXCL8 | CCL3 | CCL4 | IL-10 | ||||||
Cell type | p | r2 | p | r2 | p | r2 | p | r2 | p | r2 |
CD15+ | - | - | - | - | - | - | 0.04 | 0.42 | 0.02 | 0.54 |
CD14/16+ | - | - | - | - | - | - | 0.04 | 0.47 | - | - |
CD14/36+ | - | - | - | - | - | - | 0.03 | 0.44 | - | - |
CD14/HLA-DR+ | 0.001 | 0.69 | 0.003 | 0.63 | - | - | 0.0004 | 0.77 | 0.0001 | 0.89 |
Preterm infants were ventilated and lavaged within 24 hours of birth and cells stained with combinations of anti-CD15, anti-CD14, anti-CD16, anti-HLA-DR, anti-CD36 or matched isotype antibodies and analysed by flow cytometry. Cytokine bead arrays were carried out on cell-free lavage and the following cytokines were measured: IL-1β, CXCL8, CCL3, CCL4, and IL-10. Correlations of cytokine levels and absolute numbers of cells were analysed by linear regression and significant correlations are shown in the table.