| Primer Intended Use | Primer Name | Sequence (5'-3') |
| Insertion-deletion mutagenesis | ||
| Amplification of sp_0149 + 5' and 3' flanking region | 382 385 | 5'-CTACTACTAGAATTCATGCTGAACACACGGACAAC-3' 5'-AACCTTCCAAGCTGCAGCCGCTCCCTCCATGATAAAG-3' |
| Inverse PCR of sp_0149 + 5' and 3' flanking region (pGEMTeasy) | 383 384 | 5'-ATCATCATCATCGAAGCTTAGCCAAACCT GCGACTGTAG-3' 5'-ACTCACTCACTGAAGCTTATCGCAGCTTA CCACACAGA-3' |
| Antibiotic cassette amplification | ||
| erythromycin (ermB) | ermB_105 ermB_106 | 5'-GATGATGATGATCCCGGGTACCAAGCTTGA ATTCACGGTTCGTGTTCGTGCTG-3' 5'-AGTGAGTGAGTCCCGGGCTCGAGAAGCTT GAATTCGTAGGCGCTAGGGACCTC-3' |
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