Figure 1. Analysis of the length of 5′UTRs from human, mouse, and rabbit YB-1 mRNAs.
A. Scheme of the experiment. Total RNA was annealed with 21 nt DNA oligonucleotide that was complimentary to the YB-1 mRNA sequence 150–170 nt downstream from the translation start codon, and the sample was treated with RNase H. The reaction products were separated by denaturing polyacrylamide gel electrophoresis, and YB-1 mRNA 5′-end fragments were detected by Northern blotting with a [32P]-labeled DNA probe. B. Results of experiment with total RNA of human cells (HeLa and HEK293) – lanes 2 and 3, rabbit cells (reticulocytes) – lane 4, and mouse cells (NIH3T3) – lane 5. As a control (lanes 1 and 6) rabbit YB-1 mRNA (GenBank, NM_001082785.1) obtained by in vitro transcription was used. Radioautograph.