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. 2014 Aug 12;9(8):e104513. doi: 10.1371/journal.pone.0104513

Figure 4. Search for alternative forms of YB-1 mRNA in the cell.

Figure 4

A. Scheme of predicted alternative YB-1 mRNA. B and C. Total RNAs from MCF7 (lanes 2 and 3) and HEK293 (lanes 4 and 5) were used in the reverse transcription reaction followed by PCR (lanes 2 and 4) or in PCR only (lanes 3 and 5) with primers a and b specific to intron 1 and exon 2 of the YB-1 gene (B) or primers c and d specific to intron 1 and exon 5 of the YB-1 gene (C). PCR products were resolved by electrophoresis in 2% agarose gel stained with ethidium bromide. Lane 1 shows the DNA ladder.