Figure 1. Sequence alignment of the variable domains of the (a) Heavy chains, (b) Light chains, and (c) homology among the CDR3-H of anti-phOx germline antibody, BR55-2, BR96, B3, mu3S193 and hu3S193.

Dashes indicate identities with respect to the germline antibody sequence. Numbering corresponds to that of Kabat, CDRs are underlined. Asterisks indicate amino acid residues that participate in antigen contact based upon hu3S193 crystal structure. (d) Heavy chain and (e) Light chain amino acid sequence alignment showing the distribution of nucleotide substitution(s) -the amino acids have been color coded representative of the nucleotide substitution(s) found at that position with respect to the germline sequence– yellow: transition type substitution at any of the nucleotide positions comprising the codon, red: transversion type substitution at any of the nucleotide positions comprising the codon, pink: when both transition and transversion substitutions occur in the codon for that corresponding amino acid. Footnote: Position 77H in BR96 is Serine in its PDB entry but it corresponds to Threonine in the nucleotide sequence as reported in its US patent no: 57288211. Hence, the type of nucleotide substitution could not be determined at that position. Also, gene sequence for cr1 could not be found beyond position 94. Nucleotide substitutions beyond that were not considered.