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. 2014 Aug 12;9(8):e104323. doi: 10.1371/journal.pone.0104323

Figure 4. Activation of NF-κB signaling in HSCs in hepatic schistosomiasis.

Figure 4

(A) Total protein, cytoplasm protein and nuclear protein were extracted from HSCs and then levels of p65 were detected in each by western bolt; (B) the activity of NF-κB presented by the ratio of nuclear p65 to cytoplasm p65; and (C) the total p65 level. Expression level of (D) Ccl2; (E) Ccl3; (F) Ccl5; (G) Cxcl1; (H) α-SMA; and (I) Colα1 determined from total RNA by real-time PCR. *P<0.05, **P<0.01, ***P<0.001, compared with samples from the first day of infection (day 0).