Figure 3. Bubble diagram showing occurrences of known protein-family domains (Pfams) associated with either plasmid-selfish (a) or non-plasmid-selfish (b) functions among 1,119 genes predicted from a de novo assembly made from two wastewater metamobilomes.

The two samples were isolated with either an established method (S) or a modified method using electroelution (L; this study). Each bubble represents a cluster of Pfam-domain containing proteins and is positioned according to the average coverage of all genes encoding that protein domain against a weighted mean of LS-skew values (see methods) in which gene coverages were used as weights. For non-plasmid selfish function (b), only clusters with two or more genes were included.