Table 2. Coveragesa of model plasmidsb from spiked and non-spiked wastewater metamobilomes.
Library | pBR322 | pKJK5 | gfpmut3*-gene |
S | 150 (99.9%) | 1.4 (2.7%) | 0.0 (0%) |
L | 45,000 (100%) | 21 (96%) | 12 (100%) |
Ss | 140,000 (100%) | 3.3 (37%) | 1.0 (100%) |
Ls | 120,000 (100%) | 78 (99.5%) | 53 (100%) |
Numbers in parenthesis indicate the percentage of base positions covered by sequencing reads.
Defined as X-coverage pr. 10 million Illumina reads.
Due to a lack of a complete pKJK10-sequence, pKJK5, which covers 97% of the pKJK10-sequence, was used instead, along with the 755 bp gfpmut3*-gene, which is embedded in the Tn10-transposon inserted on pKJK10.