Figure 3. Expression of the M, H, and F proteins from peste des petitsruminants virus (PPRV) and virus-like particles (VLPs).

TheVLPs were purified using sucrose gradient centrifugation, and the protein composition was determined by western blot analysis using a mouse anti-PPRV polyclonal antibody. Lane 1, Sf21 cells (negative control);lane 2, PPRV-H VLPs; lane 3,PPRV-F VLPs; lane 4, molecular weight marker;lane 5, PPRV (positive control).