Table 1. Primer sequences used for RT-PCR amplification of M, H, and F genes of peste des petits ruminants (PPRV) and identification of recombinant bacmids.

Primers	Primers Sequence (5′–3′)	Enzyme site
M1	CCGCTCGAGGCCACCATGACCGAGATCTAC	XhoI
M2	CGGGGTACCTTACAGGATCTTGAACAG	KpnI
H1	CGGGATCCGCCACCATGTCCGCACAAAGG	BamHI
H2	CCCAAGCTTTCAGACTGGATTACATGTT	HindIII
F1	CGCGGATCCGCCACCATGACACGGGTCG	BamHI
F2	GCTCTAGACTACAGTGATCTCACGTAC	XbaI
M13 For	CCCAGTCACGACGTTGTAAAACG
M13 Rev	AGCGGATAACAATTTCACACAGG

The primers used for RT-PCR amplification of the M, H, and F genes of PPRV. The primer pairs M1/M2, H1/H2, and F1/F2 were used to amplify the M, H, and F genes, respectively. The underlined nucleotidesin the forward primers are a Kozak sequence added to optimize the expression of the target foreign gene. Restriction enzyme sites were introduced at the respective 5′-termini (shown in bold); the relevant enzymes are indicated in the last column. The primer pairs M13 For/M13 Rev were used to identify the recombinant bacmids.