Table 3. Peste des petitsruminants virus (PPRV) neutralizing antibody titers and lymphocyte proliferation responses in mice immunized with PPRV virus-like particles (VLPs) or native PPRV particles.
| Immunogen (µg) | Neutralization titer (weeks post-primary immunization) | Mean Stimulation Index* | ||
| 0 | 3 | 7 | ||
| PPRV-H VLP(10) | <4 | 8–16 | 32–64 | 1.98±0.06a |
| PPRV-H VLP(40) | <4 | 16–32 | 32–64 | 1.73±0.12 a |
| PPRV-F VLP(10) | <4 | 4–8 | 8–16 | 1.81±0.34 a |
| PPRV-F VLP(40) | <4 | 8–16 | 16–32 | 1.87±0.20 a |
| PPRV (10) | <4 | 4–16 | 32–64 | 1.87±0.23 a |
| PPRV (40) | <4 | 16–32 | 32–64 | 1.78±0.14 a |
| PBS | <4 | <4 | <4 | 1.07±0.15 b |
PPRV neutralizing antibody titers was determined in mice immunized with PPRV VLPs and PPRV vaccine virus Nig75/1, and the serum samples were collected at weeks 0, 3, and 7 following the primary immunization. Lymphocyte proliferative responses were analyzed using splenocytes collected at 1 week after boosting.
PBS = phosphate-buffered saline, as negative control; PPRV = Peste des petits virus; VLP = virus-like particles.
*Different superscript letters indicate that the differences were significant (p<0.05), same superscript letters indicate that the differences were not significant (p>0.05). (according to one-way ANOVA, using GraphPad).