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. 2014 Aug 12;9(8):e105005. doi: 10.1371/journal.pone.0105005

Figure 5. Severe hyperoxia decreases the number of hepatocytes immunostained for VEGF.

Figure 5

A) Immunohistochemical detection of VEGF expression in neonatal rat livers exposed to moderate and severe hyperoxia. a) ambient air; b) 60% hyperoxia; c) 95% hyperoxia; d) negative control; scale bar: 50 µm, magnification 40x. B) Graphic representation of the percentage of VEGF positive nuclei (±SD); densitometric analysis determined by direct visual counting of ten fields for each of three slides per sample. * = p<0.05; ** = p<0.01.