Table 1. Recombination rates and interinterval interference.
Type | Intervals | N° of Tetradsc | d (CM)d | d ratio (axr1/wt)e | NPD Ratiof | p (NPDr = 1)g |
Wt | I5aa | 3,118 | 24.2 | — | 0.27 | <10−12 |
I5ba | 3,118 | 15.5 | — | 0.37 | <10−3 | |
I5ab | 1,899 | 27.9 | — | 0.27 | <10−11 | |
I5bb | 1,899 | 16.7 | — | 0.27 | <10−3 | |
I5d | 8,947 | 8.6 | — | 0.21 | 10−5 | |
I5c | 8,947 | 8.9 | — | 0.35 | <10−3 | |
I3c | 10,245 | 4.8 | — | 0.43 | 0.1 | |
I3b | 10,245 | 17.0 | — | 0.30 | <10−18 | |
axr1 | I5aa | 1,263 | 17.0 | 0.7** | 2.69 | <10−7 |
I5ba | 1,263 | 23.7 | 1.5** | 1.63 | <10−2 | |
I5ab | 1,107 | 24.4 | 0.9* | 1.47 | 0.05 | |
I5bb | 1,107 | 29.9 | 1.8** | 1.18 | 0.05 | |
I5d | 2,274 | 9.1 | 1.1 | 0.82 | 0.9 | |
I5c | 2,274 | 8.8 | 1.0 | 0.74 | 0.7 | |
I3c | 1,499 | 4.9 | 1.0 | 1.24 | 0.9 | |
I3b | 1,499 | 20.9 | 1.2** | 1.24 | 0.4 |
Correspond to the data obtained for two independent experiments for intervals I5a and I5b.
Only four-spore viable tetrads were considered. They correspond to 97% of the tetrads (n = 3,756) in wild type and 10% of the tetrads (n = 5,973) for axr1.
Map distances were calculated using the Perkins genetic map equation [51] using raw data from Table S3B.
Genetic distance ratio between axr1 and wild type. It compares recombination rates between the two genotypes. Asterisks indicate significant differences between mutant and wild type (* p<0.05; ** p<0.01).
Ratio between the observed number of double COs (based on NPD tetrad frequency) to the expected number of double COs under the hypothesis of no interference (see Table S5). The NPDr gives the strength of interference within the considered interval (no interference if the NPDr is equal to 1, absolute interference if the NPDr is equal to 0, negative interference if the NPDr is above 1).
The p values indicate significant differences between IR and 1.