Figure 2. Chronic exposure to AS enhances the migration and invasion of HT-29 cell lines. (A–J) Wound healing rate of HT-29 cell lines at 0, 24, and 48 h after the scratch. AS treated cells, especially the AS (60), migrated much faster than control cells, Note that the wound in AS (60) was nearly closed at 48 h after the scratch (I); bars = 100 μm. (J) is the quantification of (A–I). (K–Q) Representative crystal violet staining images of cells which attached to the bottom of the transwell filter at 24 h after treatment. (K–M)are the lower power images and (N–P)are the higher power images of (K–M). (Q) The intensity of violet staining was measured as absorbance at 560 nm; bars = 100 μm in all panels. All experiments were performed 3 times independently, *P < 0.05; **P < 0.01.