Figure 3. (A–D) Cells transfected with Lenti-218 (A andC) or Lenti-NC (B andD) in regular optical vision and GFP vision (original magnification 100×). (E) Expression of miRNA-218 in cells transfected with lenti-218 or control. The relative quantitations of miRNA-218 in BxPC-3-LN group (blank control), Lenti-NC group, and Lenti-218 group were 1.007 ± 0.1466, 0.7627 ± 0.1704, and 143.5 ± 9.142 respectively. (F) The expression of ROBO1 protein in cells transfected with lenti-218 or control. A lower expressing level of ROBO1 protein was showed in Lenti-218 group compared with Lenti-NC group or Blank control. (G) Expression of ROBO1 in cells transfected with Lenti-218 or control. The relative quantitations of ROBO1 in three groups were 1.024 ± 0.2723, 0.9920 ± 0.1863, and 0.1327 ± 0.04005 respectively. P value < 0.05 was denoted as *.