Figure 4. RCAd enhanced AdIL2 transduction inhibited tumor growth. Human xenograft tumors were established by subcutaneous injection of approximately 1 × 10⁷ NCI-H460 tumor cells into the right hind limbs of mice. When tumors reached 5 mm in diameter, mice were randomly divided into 3 groups, with 8 mice in each group. The injection of AdIL2 (5 × 10⁷ pfu) alone or AdIL2 (5 × 10⁷ pfu) plus Adpx21 (1 × 10⁷ pfu) significantly inhibited tumor growth in comparison with saline injected tumors (A). In another set, 72 h after virus injection mice were sacrificed and the tumor tissues were excised and homogenized in PBS (n = 4). IL2 in the supernatant were measured by ELISA. The tumors received Adpx21 plus AdIL2 had increased IL2 expression than that received AdIL2 alone (B). Data are shown as the mean ± SD. *P < 0.05.