Figure 6. A schematic diagram of replication competent adenovirus constructed in this study. E1a-P, E1a promoter; TERT-P, the telomerase (TERT) promoter; E1a and E1b, early E1 genes of the adenovirus; IRES, internal ribosome entry site; E1b19k, gene expresses E1b-19 kDa protein; E1b55k, gene expressing E1b-55 kDa protein (A). Confirmation of E1A, E1B-19 kDa, and E1B-55 kDa protein expression by western blot. NCI-H460 cancer cells were infected with different adenoviruses at a 10 MOI (pfu/cell) for 48 h and then the cell lysates were analyzed by western blot. GAPDH (36 kDa) was used as control (B).